Abstract
From a general public health perspective, a strategy combining non-targeted and targeted lipidomics MS-based approaches is proposed to identify disrupted patterns in serum lipidome upon growth promoter treatment in pigs. Evaluating the relative contributions of the platforms involved, the study aims at investigating the potential of innovative analytical approaches to highlight potential chemical food safety threats. Serum samples collected during an animal experiment involving control and treated pigs, whose food had been supplemented with ractopamine, were extracted and characterised using three MS strategies: Non-targeted RP LC-HRMS; the targeted Lipidyzer™ platform (differential ion mobility associated with shotgun lipidomics) and a homemade LC-HRMS triglyceride platform. The strategy enabled highlighting specific lipid profile patterns involving various lipid classes, mainly in relation to cholesterol esters, sphingomyelins, lactosylceramide, phosphatidylcholines and triglycerides. Thanks to the combination of non-targeted and targeted MS approaches, various compartments of the pig serum lipidome could be explored, including commonly characterised lipids (Lipidyzer™), triglyceride isomers (Triglyceride platform) and unique lipid features (non-targeted LC-HRMS). Thanks to their respective characteristics, the complementarity of the three tools could be demonstrated for public health purposes, with enhanced coverage, level of characterization and applicability.
Highlights
While the use of anabolic compounds has been banned in livestock for more than 30 years [1], the recently updated regulatory scheme confirms such provision [2]
Are described and compared the results obtained from three methods: Non-targeted RP UHPLC-HRMS and two targeted approaches, namely LipidyzerTM and a platform focused on TG regioisomers
In the frame of a global lipidomics study, a common method is the non-targeted fingerprinting using LC-HRMS, as it allows studying a large set of lipid species without any a priori hypothesis [40], i.e., theoretically all lipids accessible to the analysis technique
Summary
While the use of anabolic compounds has been banned in livestock for more than 30 years [1], the recently updated regulatory scheme confirms such provision [2]. From a public health perspective related to the chemical safety of food from animal origin, it reaffirms the European commitment to the performance of the associated controls. In this firmly reaffirmed context, the search for ever more innovative control strategies is even more topical. Tissues collected on ractopamine (RAC) fed pigs [13] These preliminary results did not allow a thorough characterisation of the lipidome since the non-targeted methods applied lacked proper data validation or extensive lipid coverage. Characterising lipidome disruptions as a consequence of growth promoter application would allow generating new knowledge on the mechanism of action of these anabolic agents and especially discovering relevant biomarkers for more efficient screening of such practices
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
