Extending the concept of predicting fish acute toxicity in vitro to the intestinal cell line RTgutGC_suppl

Hannah Schug

doi:10.14573/altex.1905032s

Abstract

Extending the concept of predicting fish acute toxicity in vitro to the intestinal cell line RTgutGC_suppl

Highlights

Fish acute toxicity is an important criterion in chemical screening for product development and environmental hazard and risk assessment, whereby the fish acute toxicity test is conducted according to OECD TG 203 (OECD, 2019)
This study supports the concept of using cell-based assays as predictive tools for fish acute toxicity, in line with already published studies (Tanneberger et al, 2013; Natsch et al, 2018; Fischer et al, 2019)
Despite originating from different laboratories and different fish cell lines (Natsch et al, 2018; the present study), the two far available data sets for fragrances are in excellent agreement and, if combined, provide strong prediction models for fish acute toxicity without the need to use fish

Summary

Introduction

Fish acute toxicity is an important criterion in chemical screening for product development and environmental hazard and risk assessment, whereby the fish acute toxicity test is conducted according to OECD TG 203 (OECD, 2019). An in vivo test is conducted where juvenile or adult fish are exposed to the chemical for 96 h to determine the concentration that causes 50% fish lethality (LC50). This test is required for registration of any chemical produced or imported in amounts ≥ 10 tons per year in the European Union under the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (EC, 2006) legislation and requires the sacrifice of at least 42 animals. Because the gill is the primary target of waterborne toxicants and a main site of interaction for shortterm chemical exposure, the RTgill-W1 cell line from rainbow trout (Oncorhynchus mykiss) (Bols et al, 1994) was selected for this approach. L-15/ex is ideal for chemical exposure because the ab-

Methods

Results

Conclusion