Abstract
The extension of chromosomal DNA is a key technique in high-resolution gene locationsuch as extended fibre fluorescent in situ hybridization. In this paper, we propose a newmicro-device designed for on-chip integration of three functions, (1) positioningof cells, (2) extending chromosomal DNA from the cells, and (3) anchoring theextended DNA fibres. The device has a flow chamber equipped with (a) multi-phaseelectrodes to create electroosmotic flow (EOF) of variable direction, (b) a set ofmicro-fabricated pocket-like structures for cell positioning, and (c) a micro-pillar arraywhose surface is chemically modified to anchor DNA fibres. First, unidirectional EOF isinduced, by which cells are carried into the micro-pockets and positioned, one cellat each pocket. After rupturing the cells, chromosomal DNA from each cell ishydrodynamically extended by EOF. When the EOF direction is rotated through90°, the fibres are made to contact with the pillars to be anchored. Because the velocity of theEOF is controllable, the breakage of DNA during the process can be minimized. Thusimmobilized DNA fibres are suspended a few microns above the surface, allowing freeaccess of probe molecules.
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