EXTENDED-SPECTRUM BETA-LACTAMASE-PRODUCINGESCHERICHIA COLIANDKLEBSIELLA PNEUMONIAEISOLATES FROM THE URINE OF DOGS AND CATS SUSPECTED OF URINARY TRACT INFECTION IN A VETERINARY TEACHING HOSPITAL

Abstract

Extended-spectrum [Formula: see text]-lactamase (ESBL)-producing microorganisms often cause severe infections; they have only limited therapeutic alternatives and have emerged in both human and veterinary medicine. Companion animals have prolonged contact with humans and could serve as a reservoir for ESBL strains. Information regarding ESBL producers in companion animals is rather limited compared to that regarding humans. Therefore, the objective of this study was to screen for ESBL producers in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in urine samples collected from dogs and cats in which urinary tract infection was suspected in a veterinary teaching hospital in Taipei, Taiwan. In addition, bla genes, multilocus sequence typing (MLST) analysis, and antibiotic susceptibility testing were performed on these ESBL producers. From December 2011 to March 2013, disc tests of cefotaxime and ceftazidime, with and without clavulanic acid, were performed on 60 E. coli, 22 [Formula: see text]. pneumoniae, and 18 P. mirabilis samples isolated from urine; 2 E. coli and 5 K. pneumoniae samples with the ESBL phenotype were identified. CTX-M-15 was the most frequently found bla gene. CTX-M-14, SHV-11, SHV-12, and SHV-28 were also identified. MLST analysis did not detect a predominant ST type in either E. coli or [Formula: see text]. pneumoniae; nonetheless, K. pneumoniae ST15 and ST11, which are zoonotic agents of public health concern, were detected in our study. All ESBL-producing bacteria exhibited a multidrug-resistant phenotype. The occurrence of ESBL-producing E. coli and K. pneumoniae in companion animals underscores the importance of ESBL screening and epidemiological study in veterinary hospitals.