Abstract
In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and transport of pancreatic tissue in physiological solution at 4oC gives quite satisfactory results.
Highlights
In type-1 diabetes mellitus (T1DM), the destruction of pancreatic beta cells causes insulinopenia, leading to hyperglycemia and ketoacidosis unless the lack of endogenous insulin production is balanced by multiple exogenous insulin injections, which today remains the primary treatment for T1, together with regular monitoring of blood glucose levels
While exogenous insulin therapy has dramatically improved the quality of life, chronic diabetic complications develop in a substantial proportion of subjects with diabetes and generally show a progressive worsening over time
In this study we examined the effects of the duration of cold ischemia on islet yield, viability of islets and preservation of the physiological function of the islets in two groups, A and B
Summary
In type-1 diabetes mellitus (T1DM), the destruction of pancreatic beta cells causes insulinopenia, leading to hyperglycemia and ketoacidosis unless the lack of endogenous insulin production is balanced by multiple exogenous insulin injections, which today remains the primary treatment for T1, together with regular monitoring of blood glucose levels. While exogenous insulin therapy has dramatically improved the quality of life, chronic diabetic complications develop in a substantial proportion of subjects with diabetes and generally show a progressive worsening over time. Intensive insulin therapy has proven effective to delay and sometimes prevent the progression of complications such as nephropathy, neuropathy or retinopathy. It is difficult to achieve and maintain long-term in most subjects, either for compliance issues or due to the increased risk of severe hypoglycemic episodes which are generally associated with intensification of exogenous insulin therapy (Hirochito et al, 2009). Despite the efficacy of insulin therapy as a treatment for T1DM there are numerous clinical complications. These are the reasons for the extensive research into pancreatic tissue transplantation as an alternative for insulin therapy during the last decade (Ricordi, 2003)
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