Abstract
(+)-7-iso-Jasmonoyl-l-isoleucine (JA-Ile) is a lipid-derived phytohormone implicated in plant development, reproduction, and defense in response to pathogens and herbivorous insects. All these effects are instigated by the perception of JA-Ile by the COI1-JAZ co-receptor in the plant body, which in Arabidopsis thaliana is profoundly influenced by the short JAZ degron sequence (V/L)P(Q/I)AR(R/K) of the JAZ protein. Here, we report that SlJAZ-SlCOI1, the COI1-JAZ co-receptor found in the tomato plant, relies on the extended JAZ degron sequence (V/L)P(Q/I)AR(R/K)XSLX instead of the canonical JAZ degron. This finding illuminates our understanding of the mechanism of ligand perception by JA-Ile in this plant, and will inform both efforts to improve it by genetic modification of the SlCOI1-SlJAZ co-receptor, and the development of the synthetic agonists/antagonists.
Highlights
Lipid-derived (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile) is a major class of phytohormones implicated in plant development, reproduction, and the defense response of plants against pathogens and herbivorous insects[1,2]
The JAZ degron sequence is highly conserved among plant species, except for the two non-canonical degron sequences IPMQRK of FaJAZ1 found in the strawberry plant (Fragaria × ananassa), and MPIARK of EcJAZ1 found in finger millet Eleusine coracana[19,20,21]
All the TIFY sequences of the JAZ proteins of Solanum lycopersicum have been previously reported: 19 TIFY genes including 12 canonical JAZ (SlJAZ111/13) and non-canonical SlJAZ12 genes are encoded in the Solanum lycopersicum genome[23,24,25]
Summary
Lipid-derived (+)-7-iso-jasmonoyl-l-isoleucine (JA-Ile) is a major class of phytohormones implicated in plant development, reproduction, and the defense response of plants against pathogens and herbivorous insects[1,2]. Perception of JA-Ile by the COI1-JAZ co-receptor causes upregulation of JAresponsive genes, leading to the expression of JAZ (JASMONATE ZIM-DOMAIN) repressor protein –a hub of jasmonate signaling[5,6]. The TIFY domain of JAZ interacts with domain C of the adaptor protein Novel Interactor of JAZ (NINJA), the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) domain of which recruits co-repressor TOPLESS (TPL)[8]. These interactions constitute a transcriptional repression machinery consisting of the MYC2-JAZ-NINJA-TPL complex, which represses the expression of JA-responsive gene. The JAZ degron sequence is highly conserved among plant species, except for the two non-canonical degron sequences IPMQRK of FaJAZ1 found in the strawberry plant (Fragaria × ananassa), and MPIARK of EcJAZ1 found in finger millet Eleusine coracana[19,20,21]
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