Abstract
Bacillus Species was isolated from a soil sample, the isolate was cultivated, identified and its culture medium was extracted. Specific media called Gibbon’s media used for the cultivation and confirmation of the bacteria. 16S rRNA study was done for the morphological identification of the isolate. The crude which was collected from Bacillus endopyticus was purified by column chromatography. The antibacterial activity of the crude extract was identified by agar diffusion test by measuring the minimum inhibitory concentration by dilution assay. The purified extract was active against Staphylococcus aureus, Bacillus cerues, Escherichia coli. The study suggest that Bacillus Sps have the potential to produce antibiotics and can be used to control the growth in future. The further work was able to bring the microbial strains under control. The percentage of cell death was calculated the cell death was 97.95%.
Highlights
Chemical food additives are known to cause extremely deleterious effects on the human health
The various harmful effects of the chemical additives used in the food industry have been widely reviewed throughout the scientific literature by many authors
Bacillus tequilensis is known to produce a wide array of antibiotics [13] that can be effectively used against common food contaminating microorganisms
Summary
Chemical food additives are known to cause extremely deleterious effects on the human health. Bacillus subtilis as an important organism [11] An organism with both anti-microbial potential as well as a probiotic action is very valuable in the field of bio-preservation. This is because the organism can serve a two-way purpose of preserving the food material as well as enriching its nutritional composition as a probiotic. Bacillus tequilensis is known to produce a wide array of antibiotics [13] that can be effectively used against common food contaminating microorganisms. This paves the way for utilization of this bacterium as a bio-preservant
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