Expressão tecido específica do fator de inibição de migração de macrófagos (Mif) na interface materno fetal em camundongos

Abstract

FARIA, M.R. Tissue specific expression of macrophage migration inhibitory factor (Mif) at the mouse maternal fetal interface. 74 p. Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, 2009. Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the inflammatory and immune response. Recent evidence also suggests a critical role during human implantation and early embryonic development. The overall goal of this study was to characterize MIF expression by trophoblast and embryo placental cells and by decidua during mouse pregnancy. MIF was immunolocalized at implantation sites (trophoblast and decidual cells) on gestation days (gd) 7.5, 10.5, 13.5 and 17.5. Ectoplacental cones and fetal placentas dissected from the maternal tissues were used for Western blotting. Ectoplacental cones, fetal placentas and deciduas were used for qRT-PCR assays on the same gestation days. During the early post-implantation period (gd. 7.5), decidual cells and trophoblast giant cells showed strong MIF reactivity. In later placentation phases (gds 10.5–17.5), MIF appeared to be concentrated in the junctional zone on giant and spongiotrophoblast cells. At these gestational periods, decidual reactivity to the MIF antibody was weaker than early post-implantation stage. MIF protein expression at fetal placental compartment increased significantly from gd 7.5 to 10.5 (p=0.005) and from gd 7.5 to 13.5 (p=0.03). Higher mRNA expression was found on gd 10.5 and was significantly different from gds 13.5 (p=0.048) and 17.5 (p=0.009). On contrary, MIF gene expression at decidua on gd 7.5 was significantly greater than those on gds 10.5 (XXX), 13.5 (XXX) and 17.5 (XXX). The up-regulation of MIF on gd 10.5 coincides with the stage in which the placenta assumes its four-layered organization (with secondary giant cells, spongiotrophoblast, labyrinth and chorionic plate) and the fetal blood circulation begins. Altogether, this temporal tissue-specific distribution and expression data suggests that MIF may play a modulator role in the onset of placentation or in the adaptation of the placenta to the uterine environment. Moreover, it reinforces the idea that MIF is crucially important for placental interface homeostasis and successful gestation.