Abstract
to compare the expression of tumor necrosis factor-alpha (TNF-alpha) in ovular membranes with premature rupture (MPR) and with opportune rupture; to verify the association between the expression of the TNF-alpha in ovular membranes and the degree of chorioamnionitis, correlating the expression of the TNF-alpha and the membranes' time of rupture. ovular membranes from 31 parturients with MPR, with gestational ages over 34 weeks, and from parturients with opportune membranes' rupture, with gestational ages equal or over 37 weeks. Chorioamnionitis detection has been done by histopathological analysis. The evaluation of the TNF-alpha expression has been done by immune-histochemical technique, using the labile streptavidin-biotin-peroxidase (LSAB) method. the average rupture time was 16.6 hours. The ratio of the TNF-alpha expression in the Control and Study Groups did not show a significant difference (chi(2)=6.6; p=0.08). In the Study Group, there was no correlation between the degree of chorioamnionitis and the intensity of TNF-alpha expression (Spearman's coefficient (Rs)=0.4; p=0.02). there was no significant difference between the TNF-alpha expression in ovular membranes with premature or opportune rupture; in the Study Group, there was significant association between TNF-alpha expression and the degree of chorioamnionitis, and there was no association between rupture time and the intensity of TNF-alpha expression.
Highlights
PURPOSE: to compare the expression of tumor necrosis factor-alpha (TNF-α) in ovular membranes with premature rupture (MPR) and with opportune rupture; to verify the association between the expression of the TNF-α in ovular membranes and the degree of chorioamnionitis, correlating the expression of the TNF-α and the membranes’ time of rupture
CONCLUSIONS: there was no significant difference between the TNF-α expression in ovular membranes with premature or opportune rupture; in the Study Group, there was significant association between TNF-α expression and the degree of chorioamnionitis, and there was no association between rupture time and the intensity of TNF-α expression
Apesar de não ter sido notada diferença significante entre as expressões do TNF-α em membranas ovulares com ruptura prematura e com ruptura oportuna das mesmas, é fato que a pesquisa de TNF-α diretamente nas membranas ovulares pode ser utilizada nos casos de ruptura prematura de membranas (RPM) que cursam com complicações infecciosas maternas e perinatais, para melhor elucidação do real foco infeccioso nos quadros duvidosos
Summary
PURPOSE: to compare the expression of tumor necrosis factor-alpha (TNF-α) in ovular membranes with premature rupture (MPR) and with opportune rupture; to verify the association between the expression of the TNF-α in ovular membranes and the degree of chorioamnionitis, correlating the expression of the TNF-α and the membranes’ time of rupture. Face à escassez de trabalhos relacionados ao tema e ao provável envolvimento do TNF-α na presença de infecção em casos de RPM, justifica-se a realização do presente estudo, que teve como objetivos comparar a expressão do TNF-α em membranas ovulares com ruptura prematura e com ruptura oportuna das mesmas; verificar a associação entre a expressão do TNF-α em membranas ovulares e o grau de corioamnionite das mesmas, além de correlacionar a expressão do TNF-α e o tempo de ruptura das membranas.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
