Expressão da cox-2 e CD105 no câncer de mama e sobrevida livre de doença

Melina Grudzinski,Eduardo Lahude,Cláudio Galleano Zettler,Eduardo Cambruzzi,José Luiz Pedrini,Ricardo Francalacci Savaris

doi:10.1590/s0104-42302006000400031

Abstract

To verify the expression of CD105 in primary breast cancer, and the expression of cyclooxygenase-2 in primary breast cancer and in the respective axillary lymph nodes. Seventy two women from 18 to 80 years of age, with a diagnosis of Ductal Infiltrative Breast Cancer, stage II, histological type non special, with their respective axillary lymph nodes were submitted to surgical treatment at the "Hospital Nossa Senhora da Conceição" between 2001 and 2002. Immunohistochemical analyses of CD 105 in the primary breast cancer and of COX 2 in the local axillary lymph node were compared for local recurrence. Of the 72 patients with primary tumors, 40 had positive axillary lymph nodes, while 32 were negative. For each primary tumor, only one lymph node was analyzed. . Fifteen patients had local recurrence after 26 months (CI 95% 24-28). Presence of COX-2 in the primary tumors was verified in 52 cases, and presence of CD105 in 34 cases. These independent prognostic factors were not correlated to local recurrence (P= 0.203 e P=0.145, respectively). The period free of local recurrence for patients with positive expression of COX-2 in axillary lymph nodes (with metastasis or not) was of 19 months, while patients with negative expression had a 28.3 months long period free of local recurrence. The positive expression of COX-2 in axillary lymph nodes (either positive or negative) seems to be an independent prognostic factor for local recurrence.

Highlights

Seventy two women from 18 to 80 years of age, with a diagnosis of Ductal Infiltrative Breast Cancer, stage II, histological type non special, with their respective axillary lymph nodes were submitted to surgical treatment at the Hospital Nossa Senhora da Conceição between 2001 and 2002
Immunohistochemical analyses of CD 105 in the primary breast cancer and of COX 2 in the local axillary lymph node were compared for local recurrence
Presence of COX-2 in the primary tumors was verified in 52 cases, and presence of CD105 in 34 cases

Summary

DE DOENÇA

MELINA GRUDZINSKI*, EDUARDO CAMBRUZZI, EDUARDO LAHUDE, RICARDO FRANCALACCI SAVARIS, JOSÉ LUIZ PEDRINI, CLÁUDIO GALLEANO ZETTLER Trabalho realizado na Fundação Faculdade Federal de Ciências Médicas de Porto Alegre - Laboratório de Anatomia Patológica e Citopatologia – Pós-Graduação, Porto Alegre, RS. Foram incluídas na coorte inicial todas as pacientes com o diagnóstico de carcinoma ductal invasivo de mama, não especial, primário, histologicamente confirmado, submetidas a excisão adequada (margens cirúrgicas livres), com seus respectivos linfonodos axilares, sem tratamento antes da cirurgia, sem metástases à distância, estadio II, durante os anos 2001 e 2002, operadas no Hospital Nossa Senhora da Conceição, em Porto Alegre, Rio Grande do Sul. Todas as pacientes realizaram quimioterapia adjuvante e, nos casos selecionados, radioterapia, conforme as orientações do Instituto Nacional de Câncer (Inca). Todas as etapas foram feitas com a utilização de controles positivos externos, tecidos de carcinoma de mama com padrão de revelação já conhecido para COX-2 e CD105, e o controle negativo foi realizado em casos de carcinoma ductal invasivo, sem a colocação dos anticorpos primários. A positividade para expressão da COX-2 foi avaliada pela fórmula do HSCORE (HSCORE=ΣPi), onde

Tumor primário

Análise estatística e tamanho da amostra

METHODS

RESULTS

CONCLUSION