Expressions of UHRF1, P53 and their clinical significances in colon carcinoma

Abstract

Objective To investigate the expression levels and clinical significances of ubiquitin-like with PHD and ring finger domains 1 (UHRF1) and P53 in colon carcinoma. Methods The expressions of UHRF1 and P53 in 70 colon cancer tissues and 30 normal colon ones were detected by means of immunohistochemistry to analyze the correlation of these two proteins in the occurrence and development of colon carcinoma, and the relationship between their expressions and clinico-pathological factors as well as prognosis was discussed. Results The expression levels of UHRF1 and P53 in cancer tissues were significantly higher than those of cancer-adjacent tissues (87.1% vs. 56.7%, χ2=11.366, P=0.001; 64.3% vs. 6.7%, χ2=27.988, P=0.000) and showed a positive correlation between the two proteins (r=0.248, P=0.038). Both UHRF1 and P53 were associated with TNM stages (χ2=4.426, P=0.049; χ2=6.000, P=0.016) and the depth of invasion (χ2=12.553, P=0.002; χ2=4.904, P=0.036). However, they were irrelevant with the age (χ2=0.473, P=0.494; χ2=0.090, P=0.799) and gender (χ2=2.297, P=0.166; χ2=0.512, P=0.617) of patients, as well as the size (χ2=0.638, P=0.481; χ2=2.392, P=0.215) and histological grading of tumors (χ2=2.088, P=0.352; χ2=0.303, P=0.859). Kaplan-Meier analysis showed that the median survival time of patients with UHRF1, P53 positive expression was 21.83 months that was lower than patients with UHRF1 positive expression of 24.49 months (Z=-0.624, P=0.533). Both former of which was distinctly lower than that of negative ones of 37.33 months (Z=-2.856, P=0.004; Z=-2.694, P=0.007). Conclusion Both UHRF1 and P53 are highly expressed in colon cancer tissues, which imply that UHRF1 and P53 may be strongly related with colon cancer development and can be a predictor for colon cancer prognosis. Key words: Colon neoplasms; Tumor suppressor protein p53; Ubiquitin-like with PHD and ring finger domains 1