Expressions of RECK, MMP-2 and MMP-9 and invasion of tumor metastasis in neuroblastoma

Abstract

Objective To explore the expressions of RECK, MMP-2 and MMP-9, and to examine the occurrence, development, invasion and metastatic mechanism of neuroblastoma. Methods The in vivo expressions of RECK, MMP-2 and MMP-9 were detected in tumor tissues.And PV-6000 immunohistochemistry was employed for detecting the expression levels in 35 samples of local in situ tumor and metastatic neuroblastoma tissues.The in vitro expressions of RECK, MMP-2 and MMP-9 were detected in tumor tissues.The model of nude mice was established.Then fresh specimens of neuroblastoma tissues were collected and hybridized by Oligo Tumor Metastasis Microarray of SuperArray.The luminescent signals of neuroblastoma tissues were detected and differentially expressed genes analyzed by images and data. Results The positive expression rate of RECK protein was 6.7% in metastatic neuroblastoma tissues and it significantly decreased as compared with in situ neuroblastoma tissues.The inter-group differences were statistically significant (P=0.018). The positive expression rates of MMP-2 and MMP-9 proteins were 86.7% and 73.3% respectively and both significantly rose in metastatic neuroblastoma tissues.The inter-group differences were statistically significant (P 2 folds in metastatic neuroblastoma tissues included MMP-2, MMP-3, MMP-9, and gene down-regulated by >2 folds included RECK. Conclusions As the reference indices of invasion and prognosis of neuroblastoma, RECK, MMP-2 and MMP-9 may serve as predictors of neuroblastoma and intervention targets.This study provides important guidance in the diagnosis and treatment of neuroblastoma. Key words: Neuroblastoma; Matrix metalloproteinase 2; Matrix metalloproteinase 9; Immunohistochemistry