Expressions of MVD, VEGF, Ki67 in residual prostate cancer after cryoablation

Abstract

OBJECTIVE To analyze the effects of cryoablation on the mice bearing Rm-1 prostate cancer through detecting tumor angiogenesis and cancer cell proliferation in the mice after cryoablation, and to explore the effects of cryoablation on vascular endothelium growth factor (VEGF), Ki67 protein expression and microvessel density (MVD) in the mice bearing prostate cancer. METHODS Sixty Rm-1 mouse models of prostate cancer were established．Experimental mice were randomized into 2 groups: the cryoablation group (n = 30) and the control group (n = 30). After the therapy, tumor tissues of the mice in group A and B were obtained at day 0 (without cryoablation), 1st, 3rd, 5th, 7th, 14th day, respectively, aĞ er cryoablation, and the expressions of MVD, VEGF and Ki67 proteins were detected at the same time points． RESULTS The expressions of MVD, VEGF and Ki67 proteins in group A were decreased．The lowest values of the factors were detected on the 3rd day after cryoablation, and increased slowly after that. The expressions of MVD, VEGF and Ki67 proteins in the control group were not changed. Significant changes of the expressions of MVD, VEGF and Ki67 proteins in the group A were found at different time points. Correlation analysis suggested a positive correlation between the expressions of VEGF and MVD proteins ( r = 0.8793), a positive correlation between the expressions of Ki67 and MVD proteins ( r = 0.7614), and a positive correlation between the expressions of VEGF and ki67 proteins ( r = 0.6921). CONCLUSION After argon-helium cryoablation treatment for the mice bearing prostate cancer, the expressions of MVD, VEGF and Ki67 proteins in local tumor were reduced on the 1st day. The lowest values of the factors were detected on the 3rd day after cryoablation, and then increased aĞ er that. Cryoablation combined with other modalities of treatment may effectively improve the treatment eff ects of cryoablation for prostate cancer.