Expressions of intercellular adhesion molecule-1 and monocarboxylate transporters 1 and their correlation in glioblastoma tissues

Abstract

Objective To investigate the protein and mRNA expressions of intercellular adhesion molecule-1 (ICAM-1) and monocarboxylate transporters 1 (MCT1) in human gliomas and their correlation. Methods Fifty-eight brain glioma specimens, performed excision and conformed by pathology in our hospital from March 12, 2012 to October 30, 2015, were chosen in our study; in them, 31 were low-grade gliomas (WHO I-II) and 27 were high-grade gliomas (WHO III-IV); another 10 normal brain tissues, collected during the ventriclostomy at the same period were chosen as controls. The mRNA and protein expressions of ICAM-1 and MCT1 were detected by reverse transcription-PCR and two-step immunohistochemical staining (SP method). The relation between ICAM-1 and MCT1 expressions was analyzed by statistical method. Results (1) The ICAM-1 and MCT1 mRNA expressions in normal brain tissues, low-grade tissues and high-grade tissues were increased in sequence, with significant differences (P<0.05); (2) In normal brain tissues, low-grade tissues and high-grade tissues, the positive rate of ICAM-1 protein expression was 2 0.0%, 64.5% and 74.1%, with significant differences (P<0.05); and that of MCT1 protein expression was 30.0%, 58.1% and 70.4%, with significant differences (P<0.05). (3) Positive correlation was noted between ICAM-1 and MCT1 mRNA expressions (r=0.701, P=0.002). Conclusion The higher the glioma malignant degrees, the stronger the protein and mRNA expressions of ICAM-1 and MCT1; they may play important roles together in glioma progress. Key words: Human glioma; Intercellular adhesion molecule-1; Monocarboxylate transporter-1