Expressions of intercellular adhesion molecule-1 and matrix metalloproteinase-2 in human gliomas and their correlation

Abstract

Objective To investigate the expressions of intercellular adhesion molecule (ICAM)-1 and matrix metalloproteinase (MMP)-2 in human gliomas and their correlation. Methods Sixty glioma samples, resected in our hospital from March 20, 2010 to May 10, 2014, were chosen in our study, and corroding to the WHO central nervous system tumor histological grading, they were divided into low-grade glioma group (grade I-II, n=26) and high-grade glioma group (grade III-IV, n= 34); other 10 normal brain tissues were used as controls. Reverse transcription-PCR and SP immumohistochemical staining were used to detect the ICAM-I and MMP-2 mRNA and protein expressions. Results (1) The ICAM-1 and MMP-2 mRNA expressions in normal brain tissues, lowgrade glioma group and high grade glioma group were increased in sequence, with significant difference (P<0.05); the positive rate of ICAM-1 protein expression in normal brain tissues, low-grade glioma group and high grade glioma group was 20%, 46.1% and 88.2%, with significant differences (P<0.05), and that in MMP-2 protein expression was 10%, 50% and 91.1%, with significant differences (P<0.05). The mRNA expressions of ICAM-1 and MMP-2 in gliomas were positively correlated (r=0.702, P= 0.001). Conclusions The expressions of ICAM-1 and MMP-2 are positively related to the malignant degrees of gliomas; the two may be useful reference factors to evaluate prognosis of human gliomas and potential targets for therapy. Key words: Human glioma; Intercellular adhesion molecule-1; Matrix metalloproteinase-2