Abstract
Vascular inflammation is a hallmark of Type‐1 and to a lesser extent, Type‐2 diabetes mellitus. We investigated the status of arterial cyclooxygenase‐2 (COX‐2), prostaglandin D and E synthases (PGDS and mPGES) and P450 2J2 proteins in healthy and streptozotocin (STZ) diabetic rats. Age‐matched male Sprague Dawley control and STZ‐diabetic rats were used. After 6 or 12 weeks STZ or vehicle, mesenteric arteries were harvested from rats, cleaned and prepared for Western blot analyses of target proteins. Serum levels of 6‐keto‐PGF1a, nitrite/nitrate (NOx), PGE2 and 8‐epi‐isoprostanes were determined with enzyme immunoassay kits. STZ‐diabetic were persistently hyperglycemia; COX‐2, mPGES proteins, 8‐epi‐isoprostanes, PGE2, 6‐keto‐PGF1a and NOx were significantly elevated versus control rats. CYP 2J and PGDS proteins were down‐regulated in diabetic versus control arteries. The significant increases in the expression of COX‐2 and mPGES proteins as well as enhanced serum PGE2 and NOx levels and reduced expressions of CYP 2J and PGDS are compatible with development of arterial inflammation. Support: University of Louisville Intramural Research Incentive Grant.
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