Expressions of annexins and epidermal growth factor receptor in pediatric middle ear cholesteatoma

Abstract

Objective To explore the expressions of annexin and epidermal growth factor receptor(EGFR) in pediatric middle ear cholesteatoma. Methods Twenty-three children with middle ear cholesteatoma and 26 children with normal skin of external auditory canal(control group) were selected from the children enrolled in the First Affiliated Hospital of Zhengzhou University from August 2014 to March 2016.The expressions of annexin A1 (AnxA1), AnxA2 and EGFR mRNA in cholesteatoma and normal tissues were analyzed by quantitative real-time PCR (qPCR). Protein expressions of AnxA1, AnxA2 and EGFR were evaluated by using Western blot and immunohistochemistry methods. Results The expressions of AnxA1, AnxA2 and EGFR mRNA were significantly increased in cholesteatoma compared with the control group(AnxA1: 4.68±1.77 vs.2.65±0.96, U=111.5, P<0.001; AnxA2: 3.89±1.00 vs.2.47±0.81, U=84.5, P<0.001; EGFR: 4.97±1.85 vs.3.50±0.95, U=153.5, P=0.004). AnxA1 and AnxA2 mRNA expressions were positively correlated with EGFR mRNA (AnxA1 and EGFR: r2=0.283 2, P=0.009; AnxA2 and EGFR: r2=0.213 5, P=0.027). Compared with the control group, protein expressions of AnxA1, AnxA2 and EGFR were markedly enhanced (AnxA1: 0.450±0.031 vs.0.320±0.026, U=102.4, P<0.001; AnxA2: 0.568±0.024 vs.0.365±0.028, U=94.6, P<0.001; EGFR: 0.397±0.021 vs.0.228±0.017, U=128.4, P<0.001). The results of immunohistochemistry analysis showed that the ratio of AnxA1, AnxA2 and EGFR positive cells were higher than those in the control group(AnxA1: 65.22% vs.38.46%, χ2=9.296, P=0.026; AnxA2: 69.57% vs.46.15%, χ2=8.378, P=0.039; EGFR: 69.57% vs.50.00%, χ2=10.574, P=0.014). Conclusions The expressions of AnxA1, AnxA2 and EGFR are upregulated in pediatric cholesteatoma, with AnxA1 and AnxA2 expressions positively correlated with EGFR. Key words: Annexin A1; Annexin A2; Epidermal growth factor receptor; Cholesteatoma; Child