Abstract
GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.
Highlights
Gibberellins (GAs) are important phytohormones and influence almost every aspect of plant growth and development [1] [2]
PCR results showed the band size was consistent with the target sequence (Figure 2(b)), indicating the pROKIIPlGA20ox-GFP vector was successfully transferred into agrobacterium
The results showed 90% transgenic plants produced specific band of about 1.1 kb (Figure 4), and the sequence detection verified the existence of the target gene, (a) which suggested that the vectors had been successfully transformed into A. thaliana
Summary
Gibberellins (GAs) are important phytohormones and influence almost every aspect of plant growth and development [1] [2]. It was found that overexpression or downexpression of GA20ox produced different effects on plant growth and development. Overexpression of AtGA20ox improved the growth of the transgenic populous by enlarging leaves, thickening stems and elongating the trunks [9]; and silencing of GA20ox from tomato pro-. Overexpression of GA20ox from cotton improved the stem growth and reduced the growth of leaves and roots of transformed poplar [11]. These results implied that GA20ox from different plant species might alter plant growth in different orientation
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