Abstract
Amino acid permease-like (AAP-like) gene plays a critical role in absorbing amino acids through roots in plants. A number of studies have been done on amino acids uptake in plants but till date there is no report about the expression of AAP gene in Phaseolus under field allied condition. The aim of this study is to measure the expression of AAP-like gene on alanine, glycine and proline amino acid uptake capacity in Phaseolus vulgaris at field relevant concentrations. Amongst three amino acids, a drastic significant increase of 63.15 fold in expression of AAP-like gene is observed in 50 µM alanine at 2 hr. At 50 µM of proline and 25 µM of alanine, AAP-like gene expression also shows high expression of 43.71 fold at 2 hr and 42.50 fold at 1 hr respectively. This study elucidated the dose dependent relationship of glycine, alanine and proline with the expression of AAP-like gene in amino acid transport in natural conditions in roots of P. vulgaris. Additionally, this research is also useful in identification of plants needing less surplus nitrogen additions and helpful in optimizing fertilizers by tailoring AAP gene expression to match plant uptake capacities in agriculture.
Highlights
Plant growth and development are dependent on the attainment and distribution of nitrogenous compounds throughout the plant body
The aim of this study is to measure the expression of AAPlike gene on alanine, glycine and proline amino acid uptake capacity in Phaseolus vulgaris at field relevant concentrations
The solution in excess was blotted with tissue paper after dipping third time and right away the roots of intact plants were allowed to submerge in 25 ml of a solution of glycine, alanine and proline at a 25 μM and 50 μM concentration separately for 2 hr in 50 ml vials
Summary
Plant growth and development are dependent on the attainment and distribution of nitrogenous compounds throughout the plant body. Several compounds essential to plant development that includes nucleotides, hormones, chlorophyll and secondary metabolites are synthesized from amino acids [1]. Amino acids are the building block elements for enzymes and proteins that make the skeleton and give fuel through metabolism to the plant. Plants absorb amino acids right from the soil in the form of nitrate and ammonium and thereby assimilate them to amino acids [2, 3]. Most of the amino acids are synthesized in plastids, cytosol, mitochondria and peroxisomes of roots and leaves and available immediately to metabolic processes. Compartmentalization and proper channelization of the amino acids throughout plant body are performed by several transporters present in the membrane [4,5,6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
