Abstract
Atherosclerotic lesions of the coronary arteries are still in charge of significant annual morbidity and mortality despite intense therapeutic advancements. Genome-born elements contribute substantially to the atherosclerosis process. ANRIL is one of the long non-coding RNAs with outstanding functions particularly regulation of genes involved in atherosclerosis development. In this study, we measured ANRIL expression (circular-, linear-, and circular/linear ratio) in hypertensive patients with coronary artery disease (CAD) compared with peers without CAD. Among hypertensive patients who were candidates of angiography, 25 subjects with CAD and the equal number without CAD were considered as the case and control groups, respectively. Different categories of data were recorded through a predefined questionnaire. Before angiography, blood samples were obtained. After RNA extraction and cDNA synthesis, quantitative PCR was performed using specific primers for circular and linear ANRIL. Age and gender were not different between the groups. Most of the parameters of the lipid profile besides creatinine and blood urea nitrogen were remarkably worse in the case group. Circular ANRIL was significantly lower in the case group while linear counterparts were significantly higher in this group. Circular/linear ratio was also significantly lower in the case group. To overcome growing devastating trend of CAD, scrutinizing different factors involved in the initiation and development of atherosclerosis is a must. Atheroprotective role of circular ANRIL and atheroprogressive role of linear ANRIL were shown in our patients with hypertension.
Highlights
The greatest share in cardiovascular toll has been allocated to coronary artery disease (CAD)[1]
Genome wide association studies demonstrated that atherosclerotic vascular disease, CAD, stroke, myocardial infarction, and aortic aneurysm are all associated with chromosome 9 p2111–15
Our hypothesis is that there might be a difference in the expression level of circular and linear ANRIL as well as circular/linear ratio in hypertensive patients with CAD compared with non-CAD peers
Summary
The greatest share in cardiovascular toll has been allocated to coronary artery disease (CAD)[1]. Harboring a wealth of information revealed their potential for regulation of different mechanisms One type of these untranslated elements is long non-coding RNAs (lncRNAs) with more than 200 nucleotides long[4]. Genome wide association studies demonstrated that atherosclerotic vascular disease, CAD, stroke, myocardial infarction, and aortic aneurysm are all associated with chromosome 9 p2111–15 This type of association is independent of traditional risk factors like hypertension, obesity, smoking, or lipid profile disorders. An association between ANRIL levels and hypertension was r eported[30] In one study, both ANRIL and hypertension were shown to be independent risk factors for restenosis in CAD patients, and interaction between ANRIL and hypertension was statistically significant[31]. Our hypothesis is that there might be a difference in the expression level of circular and linear ANRIL as well as circular/linear ratio in hypertensive patients with CAD compared with non-CAD peers
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