Abstract
Objective To express and purify of the FAM172A2 recombinant protein,and to prepare the FAM172A2 specific rabbit polyclonal antibody.Methods FAM172A2 cDNA (1113 bp) was obtained by reverse transcription-polymerase chain reaction (RT-PCR).The prokaryotic expressive vector was constructed.The protein expression was induced and the protein was analyzed with Western blotting.The purified pET-32a( + )-FAM172A2 fusion protein was used to gain polyclonal antibody.The specificity and potency of polyclonal antibody were evaluated by Western blotting and enzyme linked immunosorbent assay (ELISA).Results The FAM172A2 fusion protein was highly expressed.The protein was mainly in the inclusion body.ELISA indicated the titer of polyclonal antibody ＞ 1:160 000.The high specificity was confirmed with Western blotting.Conclusion The recombinant FAM172A2 fusion protein and the antiFAM172A2 polyclonal antibody will be valuable tools for the investigation on the biological function of FAM172A2. Key words: Prokaryotic expression; Protein purification
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