Abstract
The prokaryotic expression plasmid of hygromycin-B-phosphotransferase(HPT) was constructed.The hpt gene was cloned by PCR.It was digested by SalⅠ/NotⅠand subcloned into expression vector pET-28b(+).pET-28b-hpt was transferred into E.coli Rosset;fusion protein was induced by isopropyl-β-D-thiogalactopyranoside(IPTG) and analyzed by SDS-PAGE.The most products existed in an inclusion body form.The HPT protein purified by Ni2+-NTA column was used to immunize New Zealand rabbits.The HPT polyclonal antibodies reveal high sensitivity and specificity.
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