Abstract
BackgroundLily symptomless virus (LSV) is widespread in many countries where lily are grown or planted, and causes severe economic losses in terms of quantity and quality of flower and bulb production. To study the structure-function relationship of coat protein (CP) of LSV, to investigate antigenic relationships between coat protein subunits or intact virons, and to prepare specific antibodies against LSV, substantial amounts of CP protein are needed.ResultsThus, full-length cDNA of LSV coat protein was synthesized and amplified by RT-PCR from RNA isolated from LSV Lanzhou isolate. The extended 33.6 kDa CP was cloned and expressed prokaryoticly and then purified by Ni-ion affinity chromatography. Its identity and antigenicity of recombinant CP were identified on Western-blotting by using the prepared anti-LSV antibodies.ConclusionsThe results indicate that fusion CP maintains its native antigenicity and specificity, providing a good source of antigen in preparation of LSV related antibodies. Detailed structural analysis of a pure recombinant CP should allow a better understanding of its role in cell attachment and LSV tropism. This investigation to LSV should provide some specific antibodies and aid to development a detection system for LSV diagnostics and epidemiologic surveys.
Highlights
Symptomless virus (LSV) is widespread in many countries where lily are grown or planted, and causes severe economic losses in terms of quantity and quality of flower and bulb production
The expected 900 bp cDNA encoding Lily symptomless virus (LSV) coat protein (CP) was amplified by RT-PCR from isolated RNA
The results indicate that fusion CP maintains its native antigenicity and specificity, providing a good source of antigen in preparation of LSV related antibodies
Summary
Symptomless virus (LSV) is widespread in many countries where lily are grown or planted, and causes severe economic losses in terms of quantity and quality of flower and bulb production. Symptomless virus (LSV; family, Genus Carlavirus, species) is the most prevalent virus infecting Lanzhou lily [2], and it has been reported in USA, Europe, Australia and Asia [3,4,5,6,7] It is one of the most harmful viruses of lilies that causes severe losses in terms of quantity as well as quality of bulb and flower production[8]. The host range of LSV is mostly distributed in genus Lilium, in one case reported in Alstroemeria[9] The observed abnormalities such as growth reduction, smaller flowers and lower bulb yield can be caused by combined infection with LSV and Chinese Academy of Sciences, Lanzhou 730000, China cucumber mosaic virus (CMV) [8] which threatens the yield and commercial production of lily plants
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