Abstract
E. coli is the main causative agent of mastitis in dairy cows, but the mechanism of molecular regulation underlying the occurrence and development of mastitis has not yet been fully elucidated. In this study, an E. coli-induced mastitis model was created and RNASeq technology was used to measure the miRNA expression profiles at different times post-infection (0, 1, 3, 5, 7 dpi), as well as to screen for differentially expressed miRNA. The results show detection of 2416 miRNAs, including 628 known miRNAs and 1788 newly discovered miRNAs. A total of 200 differentially expressed miRNAs were found at different time points. Bioinformatics analysis showed that these differentially expressed miRNAs may regulate the occurrence and development of mastitis in dairy cows through seven signal transduction pathways, namely cytokine-cytokine receptor interaction, MAPK signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, T cell receptor signaling pathway, Toll-like receptor signaling pathway, and cell adhesion molecules. In addition, bta-miR-200a, bta-miR-205, bta-miR-122, bta-miR-182 and the newly discovered conservative_15_7229 might be involved in immune process in late stage of E. coli-induced mastitis. The results of this study lay the foundation for molecular network analysis of mastitis and molecular breeding of dairy cows.
Highlights
Mastitis is an inflammatory disease caused by pathogenic microorganisms in mammary tissues
There have only been a few reports on blood miRNAs, for example, in the use of high-throughput techniques to detect miRNA expression profiles in clinical mastitis in dairy cows[14,15] and in the detection of miRNA expression levels in mononuclear cells from dairy cows with mastitis caused by infection with S. uberis[16]
Dairy cow mastitis is an inflammatory disease caused by pathogenic microorganisms
Summary
Mastitis is an inflammatory disease caused by pathogenic microorganisms in mammary tissues. E. coli is one of the most commonly observed pathogens in dairy cow mastitis. There have only been a few reports on blood miRNAs, for example, in the use of high-throughput techniques to detect miRNA expression profiles in clinical mastitis in dairy cows[14,15] and in the detection of miRNA expression levels in mononuclear cells from dairy cows with mastitis caused by infection with S. uberis[16]. The changes in expression of blood miRNA in dairy cows caused by E. coli infection have not been previously reported. In this study, RNAseq was used to determine the expression levels of blood miRNA in E. coli-induced mastitis at different times after infection, in order to provide a basis for the biotherapy, and molecular mechanism behind mastitis in dairy cows. Mapped Small dpi Raw reads Q30 (%) Clean reads RNA Reads dpi Clean reads rRNA snRNA snoRNA
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