Abstract
In recent years, advancements in genome-wide analyses of the mammalian transcriptome have revealed that long noncoding RNAs (lncRNAs) is pervasively transcribed in the genome and an increasing number of studies have demonstrated lncRNAs as a new class of regulatory molecules are involved in mammalian development (Carninci et al. (2005); Fatica and Bozzoni (2014)), but very few studies have been conducted on the potential roles of lncRNAs in mammalian testis development. To get insights into the expression patterns of lncRNA during mouse testis development, we investigated the lncRNAs expression profiles of neonatal and adult mouse testes using microarray platform and related results have been published (Sun et al., PLoS One 8 (2013) e75750.). Here, we describe in detail the experimental system, methods and validation for the generation of the microarray data associated with our recent publication (Sun et al., PLoS One 8 (2013) e75750.). Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE43442.
Highlights
SpecificationsRaw data: TXT files, normalized data: SOFT, MINIML, and TXT TXT files Neonatal (6-day-old) and adult (8-week-old) C57/BL6 mouse testis
In recent years, advancements in genome-wide analyses of the mammalian transcriptome have revealed that long noncoding RNAs is pervasively transcribed in the genome and an increasing number of studies have demonstrated lncRNAs as a new class of regulatory molecules are involved in mammalian development (Carninci et al (2005); Fatica and Bozzoni (2014)), but very few studies have been conducted on the potential roles of lncRNAs in mammalian testis development
Raw and processed microarray data have been deposited to the Gene Expression Omnibus (GEO) database in the National Center for Biotechnology Information (NCBI), and can be accessed by the GEO accession number, GSE43442
Summary
Raw data: TXT files, normalized data: SOFT, MINIML, and TXT TXT files Neonatal (6-day-old) and adult (8-week-old) C57/BL6 mouse testis. Microarray comparison was preformed to identify genes differentially expressed in neonatal (6-dayold) and adult (8-week-old) mouse testis N/A C57/BL6 mouse, Shanghai, China
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