EXPRESSION PROFILING OF FSH RECEPTOR (FSH-R) AND DOPAMINE RECEPTOR (D-R) GENES OF INDIAN CATFISH, CLARIUS MAGUR (HAMILTON, 1822)

Abstract

The Gene expression analysis provides a better understanding of the biological processes like Maturation; Reproduction in which the genes involved in complex regulatory networks of the physiological processes. Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most specific, precise and also easy to operate technique to examine the target genes’ expression. Expression profiles of magur FSH receptor (FSH-R) and dopamine receptor (D-R) genes were examined in the brain and gonads of premature and mature, 6 and 16 h post-injection with Ovatide TM of both sexes. Magur D-R registered highest level of expression in the brain at 6h post-Ovatide injection followed by matured brain and brain at 16h post Ovatide in male fish. C. magur FSH-R registered highest expression levels in the male brain at 6h postOvatide injection followed by the female brain at 16h post Ovatide. The study showed that D-R is mainly expressed in the mature male brain and FSH-R is also expressed in mature male brain. Ovatide TM injection for induced spawning resulted in significant increase in D-R and FSH-R gene expression in the male brain at 6 hpi.