Abstract
BackgroundGiant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA‐induced silencing complex.MethodsMicroarray was conducted on three groups of tumor tissues and normal tissues from patients with GCTB, and results showed different expression profiles of miRNAs with Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis. The functions of miR‐187‐5p and miR‐1323, which were highly expressed in GCTB, were examined by 5‐ethynyl‐2′‐deoxyuridine (EDU), transwell, and CCK8 assays. RNAhybrid et al. (rna prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments.ResultsmiR‐187‐5p and miR‐1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Luciferase assay confirmed that the two microRNAs can bind to the 3′ untranslated regions (UTR) of mRNA of FRS2. And, rescue experiments verified the relationships between the two microRNAs and FRS2.ConclusionThere were some different‐expressed microRNAs between GCTB and normal tissues. miR‐187‐5p and miR‐1323 can regulate the biological functions of GCTB through influencing the expression of FRS2.
Highlights
Giant cell tumor of bone (GCTB) is a type of borderline tumor.[1,2] It has low morbidity but high recurrence, so well as high metastasis rate, especially translocating to the lung
We considered that the two microRNAs may function in GCTB
Even though Denosumab has been approved to treat for GCTB,[5] there exists a need to demonstrate the mechanism of occurrence and development of the tumor in order to find more effective molecules functioning in GCTB
Summary
Giant cell tumor of bone (GCTB) is a type of borderline tumor.[1,2] It has low morbidity but high recurrence, so well as high metastasis rate, especially translocating to the lung. Giant cell tumor of bone (GCTB) is a type of borderline tumor.[1,2]. It has low morbidity but high recurrence, so well as high metastasis rate, especially translocating to the lung. Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. RNAhybrid et al (RNA prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments. Results: miR-187-5p and miR-1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Conclusion: There were some different-expressed microRNAs between GCTB and normal tissues. miR-187-5p and miR-1323 can regulate the biological functions of GCTB through influencing the expression of FRS2
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