Abstract
As a highly conserved protein, the translationally controlled tumor protein (TCTP) carries out vital roles in various life processes. In rubber tree, two TCTP genes, HbTCTP and HbTCTP1, were cloned, but only HbTCTP1 was studied in details. In this study, cis-acting regulatory elements, expression patterns, subcellular localization, interacting proteins, and antioxidant activity of HbTCTP were systematically analyzed. Besides the common cis-acting regulatory elements, HbTCTP promoter also harbored various known cis-elements that respond to hormone/stresses. Being consistent with the aforementioned results, HbTCTP was regulated by drought, low temperature, high salt, ethylene (ET), wounding, H2O2, and methyl jasmonate (MeJA) treatments. HbTCTP was expressed throughout different tissues and developmental stages of leaves. In addition, HbTCTP was associated with tapping panel dryness (TPD). HbTCTP was localized in the membrane, cytoplasm and the nucleus, and interacted with four proteins rubber elongation factor (REF), 17.5 kDa heat shock family protein, annexin, and REF-like stress related protein 1. Being similar to HbTCTP1, HbTCTP also indicated antioxidant activity in metal-catalyzed oxidation (MCO) system. Our results are useful for further understanding the molecular characterization and expression profiles of HbTCTP, but also lay a solid foundation for elucidating the function of HbTCTP in rubber tree.
Highlights
The translationally controlled tumor protein (TCTP) is a highly conserved protein, and it is widely expressed in all eukaryotic organisms (Bommer and Thiele, 2004)
Besides the common cis-acting regulatory elements including TATA boxes and CAAT boxes, the HbTCTP promoter harbored various known cis-elements associated with hormone response [ethylene, abscisic acid (ABA), gibberellin (GA), salicylic acid (SA), and auxin responses], stress response, as well as stress and hormone responses
The results indicated that HbTCTP expression might be involved in stress/hormone response
Summary
The translationally controlled tumor protein (TCTP) is a highly conserved protein, and it is widely expressed in all eukaryotic organisms (Bommer and Thiele, 2004). Since Yenofsky et al (1983) reported the first TCTP in mouse, the TCTPs have been cloned and characterized in different organisms. The TCTPs were reported to be associated with several biological processes such as cell growth, cell cycle progression, differentiation, malignant transformation, protection against various stress conditions and apoptosis (Amson et al, 2013). The studies on plant TCTPs are very limited. The first plant TCTP gene was cloned in Medicago sativa (Pay et al, 1992). TCTP genes have been isolated from different plants species. Besides being related to plant growth and development (Lu et al, 2007; Brioudes et al, 2010; Nakkaew et al, 2010; Qin et al, 2011), TCTPs were involved in a wide
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