Expression profile of Ki-67 in OSMF and its possible correlation with clinical and histological features

Abstract

Oral submucous fibrosis(OSMF) is a potentially malignant disorder (PMDs) with characteristic epithelial and connective tissue changes and regarded as possessing a high degree of malignant potential. Epithelial dysplasia is an important marker of malignant development from PMDs. Because agreement among oral pathologists is poor regarding lesional diagnosis, Ki-67 as a proliferative marker may have a place in objectively characterizing dysplasia in tissue specimens. The study groups included 60 patients diagnosed with OSMF based on history and clinical examination. After obtaining the details in regard to habits and clinical manifestations, the subjects were divided into Group IA and Group IB (very early and early stages-Group I A, moderate and advanced stages-Group I B). 30 subjects without an OSMF-negative control group and 30 patients of SCC-positive control. Biopsy was taken and subjected for H&E staining and IHC analysis of Ki-67. The mean count of Ki-67 was determined in OSMF cases with dysplasia and without dysplasia by using t test, mean value of Ki-67 in with dysplasia (37.41) was higher than that of without dysplasia (28.41) with no significant difference (p value = 0.0810).