Abstract
BackgroundCircular RNAs (circRNAs) is a newly discovered type of non-coding RNA, the abnormal expression of which has been demonstrated in many types of human tumors. So they have been considered as promising candidates as diagnostic and therapeutic targets in cancer. This research aimed to screen the profile of circRNA expression in salivary adenoid cystic carcinoma (SACC).MethodsUsing the threshold of FDR < 0.05 and fold change > 2 or < 0.5, 5 up-regulated and 26 down-regulated circRNAs were identified. The reliability of sequencing was verified by the expression detection of randomly selected circRNAs via qRT-PCR.ResultsMoreover, the circRNA-miRNA system was established by bioinformatics approaches and successfully identified an interaction between circRNA ABCA13 and a cancer-related miRNA (miR-138-5p), which was also verified by qRT-PCR. Moreover, the predicted molecular interaction proved that circRNA ABCA13 may promote SACC through inhibition of miR-138-5p.ConclusionsCollectively, this study has offered the first report about the circRNA expression profile and circRNA-miRNA network in SACC. All of the above could benefit the exploration of novel therapeutic target in SACC treatment.
Highlights
Circular RNAs is a newly discovered type of non-coding RNA, the abnormal expression of which has been demonstrated in many types of human tumors
Many studies concentrated in seeking biomarkers to improve the clinical prognosis of salivary adenoid cystic carcinoma (SACC), but the results showed that current chemotherapy and molecular targeting drugs can only achieve temporary partial response or stable disease in advanced SACC with local recurrence or distant metastasis [6, 7]
Identification of differentially expressed Circular RNA (circRNA) Our group performed the high-throughput sequencing for evaluating circRNA expression in the SACC samples (T group) in comparison with the matched normal samples (G group)
Summary
Circular RNAs (circRNAs) is a newly discovered type of non-coding RNA, the abnormal expression of which has been demonstrated in many types of human tumors. They have been considered as promising candidates as diagnostic and therapeutic targets in cancer. The abnormal expression of various noncoding RNAs including lncRNAs and miRNAs has been demonstrated to be closely correlated to the pathogenesis of human cancers [12,13,14]. This study aimed to identify novel circRNA biomarkers by recognizing SACC-related abnormal expression of circRNA by generation RNA sequencing analysis. The further analysis of the relationship between these circRNAs and their potential association with miRNAs could further promote our understanding of the SACC pathogenesis and propose potential therapy targets for SACC treatment
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