Expression profile of candidates immune genes in patients after stem cell transplantation

Abstract

Event Abstract Back to Event Expression profile of candidates immune genes in patients after stem cell transplantation Petra Klevcova1*, Frantisek Mrazek1, Regina Fillerova1, Zdenka Navratilova1, Antonin Vitek2, Michael Doubek3 and Martin Petrek1 1 Palacky University, Czechia 2 Institute of Hematology and Blood Transfusion, Czechia 3 The University Hospital Brno, Czechia Graft-versus-host disease (GVHD) is the most severe complication associated with allogeneic stem cell (aHSC) transplantation. There is a little information on cell profile and surface cellular markers that play important role in GVHD. The aim of this study was therefore to determine expression profile of activating markers that characterize Th1 and Th2 cell profile (TBX21 and GATA3 respectively), and surface integrins of lymphocytes (ITGA4 and ITGB7). Peripheral blood samples were taken from the 16 patients at 7 events (before chemotherapy, before aHSC transplantation, 14, 21, 28, 100 and 180 days after aHSCT). Real time PCR was used to measure mRNA relative expression of candidate genes (TBX21, GATA3, ITGA4 and ITGB7). Chemotherapy before aHSC transplantation decreased the relative expression of ITGB7 and GATA3 (p = 0,003; p = 0,0001). The number of TBX21 mRNA transcripts was up-regulated by chemotherapy (p = 0,015) and reduced after transplantation (p = 0,035). The mRNA expression of ITGB7 and GATA3 restore to state before chemotherapy in 100 and 180 days after transplantation). By contrast, TBX21 expression was increased in 100 and 180 days after aHSC transplantation compared to that before chemotherapy (p = 0,005; p = 0,022). Expression of ITGA4 was not affected within the study period. In conclusion, our pilot data indicate that chemotherapy increase Th1 marker (TBX21) and decreases the Th2 marker (GATA3) and integrin ITGB7. Further, TBX21 is reduced by aHSC transplantation and increases subsequently. Grant support: IGA MZ ČR NT12454, IGA UP 2013_009 a CZ.1.05/2.1.00/01.0030. Keywords: aGVHD, Real-Time PCR, Hematopoietic Stem Cell Transplantation, TBX21, GATA3 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Klevcova P, Mrazek F, Fillerova R, Navratilova Z, Vitek A, Doubek M and Petrek M (2013). Expression profile of candidates immune genes in patients after stem cell transplantation. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00260 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Petra Klevcova, Palacky University, Olomouc, Czechia, petra.klevcova@seznam.cz Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Petra Klevcova Frantisek Mrazek Regina Fillerova Zdenka Navratilova Antonin Vitek Michael Doubek Martin Petrek Google Petra Klevcova Frantisek Mrazek Regina Fillerova Zdenka Navratilova Antonin Vitek Michael Doubek Martin Petrek Google Scholar Petra Klevcova Frantisek Mrazek Regina Fillerova Zdenka Navratilova Antonin Vitek Michael Doubek Martin Petrek PubMed Petra Klevcova Frantisek Mrazek Regina Fillerova Zdenka Navratilova Antonin Vitek Michael Doubek Martin Petrek Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.