Abstract
Polyploidization often leads to “transcriptome shock,” and is considered an important factor in evolution of species. Analysis of the cell cycle, which is associated with survival in polyploidy, has proved useful in investigating polyploidization. Here, we used mRNA sequencing to investigate global expression in vitro (in cultured cells) and in vivo (in fin and liver tissues) in both the diploid and tetraploid Carassius auratus red var.. Differential expression (DE) of genes in diploid (7482, 36.0%) and tetraploid (3787, 18.2%) states suggested that in vitro and in vivo conditions dramatically change mRNA expression levels. However, of the 20,771 total shared expressed genes, 18,050 (87.0%), including 17,905 (86.2%) non-differentially expressed genes (DEGs) and 145 (0.7%) DEGs between diploids and tetraploids, showed the same expression trends in both cultured cells and liver tissues. Of the DEGs, four of seven genes in the cell cycle pathway had the same expression trends (upregulated in diploids and tetraploids) in both cultured cells and liver tissues. Quantitative PCR analysis confirmed the same expression trends in the nine DEGs associated with regulation of the cell cycle. This research on common characteristics between diploids and tetraploids provides insights into the potential molecular regulatory mechanisms of polyploidization. The steady changes that occur between diploids and tetraploids in vitro and in vivo show the potential value of studying polyploidy processes using cultured cell lines, especially with respect to cell cycle regulation.
Highlights
Polyploidy occurs in plants, animals, and fungi (Comai, 2005; Blomme et al, 2006)
Diploid cultured cells were obtained from the caudal fin of C. auratus red var., and tetraploid cultured cells were derived from the caudal fin of a tetraploid hybrid of C. auratus red var. (♀) × C. carpio L. (♂)
We focused on the transcriptional regulation of C. auratus red var. in vitro and in vivo to investigate whether there is a difference in cell cycle regulation
Summary
Polyploidy occurs in plants, animals, and fungi (Comai, 2005; Blomme et al, 2006). It plays an important role in the evolutionary history of species by providing a large amount of genetic material, contributing to the genomic complexity, and further promoting speciation (Comai, 2005; Blomme et al, 2006; Otto, 2007). Polyploid breeding induced by artificial and natural mutagenesis is utilized to obtain cells and organisms with genome duplication, contributing to obtaining polyploid animals to achieve high genome plasticity, including allotetraploid hybrids of Carassius auratus red var. To further study polyploid fish, the establishment of in vitro cell culture is necessary to analyze complex regulatory mechanisms including genome-wide additive and dominant expression in polyploid formation (Yoo et al, 2013)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
