Expression patterns of Th1/Th2 transcription factors in patients with guttate psoriasis

Abstract

Many lines of evidence propose that psoriasis is a T cell-mediated disease where T cell activation is followed by secretion of inflammatory cytokines. To elucidate the functional state of T cells in guttate psoriasis, we analysed mRNA expression levels of T-bet and GATA-3 for Th1 and Th2 differentiation, respectively together with Th1 (IFN-γ) and Th2 (IL-4) cytokine mRNA expression. Relative quantification of T-bet, GATA-3, IFN-γ and Th2, and IL-4 transcripts in peripheral blood leukocytes (PBL) was conducted by real-time reverse transcriptase PCR (RT-PCR). Serum levels of IFN-γ and Th2 and IL-4 were also determined by ELISA. GATA-3 and IL-4 mRNA expression levels were lower in psoriatic patients as compared to normal healthy controls. The expression levels of T-bet and IFN-γ and Th2 genes were relatively similar in the patients and controls. In addition, a marked decrease in plasma IL-4 levels was observed in the psoriasis group, while no differences were observed with regard to levels of IFN-γ and Th2 between patients and normal subjects. Furthermore, a clear correlation between decreased IL-4 mRNA expression and IL-4 (P < 0.05) was revealed. These results suggested that altered balance between Th1 and Th2 cells transcription factor genes and they products may be implicated in the pathogenesis of psoriasis.