Expression patterns of LncRNA-GAS5 and its target APOBEC3C gene through miR-103 in breast cancer patients.

Belan O Kanabe,Mustafa S Al-Attar,İbrahim Halil Kılıç,Mehmet Ozaslan,Rozhgar A Khailany,Sherwan Ahmed Aziz

doi:10.14715/cmb/2021.67.3.2

Belan O Kanabe, Mustafa S Al-Attar + Show 4 more

Open Access

https://doi.org/10.14715/cmb/2021.67.3.2

Copy DOI

Abstract

Early diagnosis of breast cancer can increase the survivability of the patients and the patient's quality of life. There is growing evidence demonstrating the active role of LncRNA-GAS5 and miR-103 in cancer biology. APOBEC enzymes are important players in immunity and may contribute to carcinogenesis. Mutation and expression alteration in the APOBEC gene family was found to have a strong correlation with breast cancer risk. This study aimed to evaluate the expression level of lncRNA-GAS5 and its target APOBEC3C in women with breast cancer through expression evaluation of miR-103. Moreover, the interaction between lncRNA-GAS5 and miR-103 was studied. In the present study, forty paired tumor and normal samples classified based on breast cancer subtypes and clinical features of patients were analyzed using gene expression studies. Immunohistochemical analysis of the gene products was performed to classify tumors. The RNA samples were extracted from breast tissue. Real-time PCR was conducted for APOBEC3C and Lnc-RNA GAS5 expression. In addition, miR-103a miScript Primer Assay was utilized for the expression of miR-103-5p. It was revealed that the expression level of APOBEC3C and lncRNA-GAS5 were significantly down-regulated; however, the miRNA-103 expression level was significantly up-regulated. GAS5 expression was positively correlated with APOBEC3C expression and negatively correlated with miR-103 expression. In conclusion, we observed down-regulation of APOBEC3C and LncRNA-GAS5 and up-regulation of miRNA 103 in breast cancer patients. The expression of GAS5 may provide a new potential treatment target for breast cancer. To clarify the role of these molecules in the cellular signaling pathways, further studies are required.

Highlights

Breast cancer is a leading debilitating and the most common cancer affecting females worldwide that shortens the life span of patients and increases mortality rate (1)
The coding region of mRNA of APOBEC3C sequence was screened to finding possible mutation via a genetic analyzer
As the most common cancer in women, breast cancer is a complex and heterogeneous tumor that arises from the breast tissue and becomes malignant (8,9)

Summary

Introduction

Breast cancer is a leading debilitating and the most common cancer affecting females worldwide that shortens the life span of patients and increases mortality rate (1). The intriguing part of cancer development is, the time manner fashion, a clone arises from the already differentiated cell due to somatic mutation, somehow, escapes from the immune surveillance mechanisms starting to cell division and proliferation and metastasis, acting like pseudo embryonic totipotent cells which lack differentiation potentials. This is not always the case, as scattered differentiated tissue fragments can be found within the proliferated mass (1)

Objectives

Methods

Results

Conclusion