Abstract
5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD 67-GFP knock-in mice. The present result showed that 5-HT 1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT 1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT 2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT 2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
