Abstract
Vitellogenin (VTG), the precursor to yolk protein vitellins (Vn), has been widely studied in oviparous animals. However, information about VTG expression during ontogenesis is limited. In this study, we investigate the developmental expression pattern of the zhikong scallop gene, Chlamys farreri vitellogenin (Cf-vtg), using in situ hybridization and real-time RT-PCR techniques. The Cf-vtg mRNA was detected in unfertilized eggs, fertilized eggs, embryos and early-stage larvae, indicating a maternally supplied Cf-vtg. It was located restrictively in D-shaped larvae, and its concentration declined at the gastrula stage and approximated zero from umbo-larva to juveniles of 4–6 mm shell height. However, it was expressed distinctly again in the hepatopancreas of both males and females with shell heights from 7 to 12 mm and in the ovary during the first gonadal development cycle. This report provides experimental evidence of the expression pattern of vtg with the maternal supplication, the hepatopancreas and male expression in C. farreri.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
