Expression Pattern of Sulf1 and Sulf2 in Chicken Tissues and Characterization of Their Expression During Different Periods in Skeletal Muscle Satellite Cells

L He,F Ye,H Xu,H Yu,Y Wang,X Zhao,Q Zhu,Y Lu,H Yin

doi:10.1590/1806-9061-2019-1165

Abstract

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. HSPGs interact with growth factors and receptors through heparan sulfate (HS) chains. The sulfation pattern of heparan sulfate chains influences signaling events mediated by heparan sulfate proteoglycans located on the cell surface. SULF1 and SULF2 are two endo-sulfatases that can cleave specific 6-O-sulfate groups within the heparan chains. To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. The SULF1 and SULF2 transcripts were widely distributed in various tissues. Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. Therefore, our findings increase our knowledge of sulfatase expression diversity and provide a solid basis for further research concerning this molecular mechanism.

Highlights

Skeletal muscles are derived from mesodermal precursor cells originating from the somites (Buckingham et al, 2003)
Serial dilutions (10-3-10-8) of the PCR products for the SULF1, SULF2 and GAPDH genes in the breast muscle tissue were tested by RT-PCR
Plotting the obtained Ct values relative to the serial dilutions of SULF1, SULF2 and GAPDH resulted in a linear correlation with square regression coefficients of 0.998, 0.998 and 0.999, respectively, suggesting that quantification of the target DNA was possible

Summary

Introduction

Skeletal muscles are derived from mesodermal precursor cells originating from the somites (Buckingham et al, 2003). Skeletal myogenesis is initiated in myogenic cells originating from the dermomyotome lips that differentiate to form primary muscle fibers (Asakura et al, 2002). Satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors (Relaix & Zammit, 2012; Maltzahn et al, 2013; Yin et al, 2013). The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals (Wang & Rudnicki, 2012; Pasut et al, 2013; Günther et al, 2013; Fry et al, 2015). Heparan sulfate (HS) structural analysis demonstrates that SULF1 and SULF2 are regulatory HS-modifying enzymes that control HS 6-O-desulfation of activated satellite cells (Houben et al, 2013; Pang et al, 2015)

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Conclusion