Expression pattern of HPV L1 in different cervical lesion tissues

Abstract

Objective To explore the factors influencing the HPV L1 protein expression in tumor tissues and to understand the expression pattern of HPV L1 protein in different cervical lesion tissues.Methods Different cervical lesion tissues were collected to detect genotypes and genome physical states of HPV 16 and 18.ELISA,Western blotting analysis,and immunohistochemical staining were used to examine the expression of HPV L1 protein in the tissues.Results A total of 48 specimens were collected,with 42 being HPV16positive and 3being HPV18 positive.In HPV16 positive specimens,25 harbored exclusively the episomal form,7carried mixed forms,and 10 had integrated type.All the HPV18 positive specimens belonged to the integrated type.The results of ELISA and Western blotting analysis displayed that HPV L1 expression decreased with the aggravation of the lesion severities.The immunohistochemistry findings showed that L1 positive reactions were of different forms and varied with different physical states of viral genome,differentiation degrees,and progression of lesion tissues.Conclusion HPV L1 expression in cervical lesion tissues has its structural foundation,and the expression levels are closely related to many factors,including the physical state of virus genome in lesion tissues,differentiation degrees of tumor tissue,and the state of virus gene sequence being inserted into host ones.