Abstract
Abstract Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia (B-ALL). Methods Relative mRNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells (PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene. Results Relative expression of BIM, BCL-6, and c-MYC mRNA in B-ALL patients was significantly lower than in healthy controls (P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission (CR) (P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups (P > 0.05). Spearman’s rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients. Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.
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