Abstract
The delayed parturition of the cloned calves has come many problems such as over-weighted offspring and death within 24 after birth. 20αhydroxysteroid dehydrogenase (20α-HSD) has a pivotal role in a regulation of the parturition time. However, little is known about a function of this gene associated with a delayed parturition in mammals. This study was performed to investigate the plasma hormonal level and the expression pattern of 20α-HSD mRNA in placentome at parturition in bovine. The blood and placentome samples were obtained from the pregnant Korean native cows by artificial insemination (control) or somatic cell nuclear transfer (SCNT). The concentration of plasma P4 level was measured using ELISA kit. Total RNA were extracted from the tissues and cDNA was synthesized. The expression of 20α-HSD mRNA was detected by semi-quantitative RT-PCR, real-time PCR, and Northern blot analysis. The plasma P4 level of control (0.1±0.22ng/ml) was dramatically reduced at parturition compared to that of SCNT (6.68±0.56ng/ml). According to RT-PCR and Northern blotting, the 20α- HSD mRNA in SCNT was highly expressed in SCNT group and the Ct value was 5.67±0.51 (control) and 0.06±0.004 (SCNT) by real-time PCR, respectively. Therefore, it can be concluded that the imbalance of plasma P4 level and 20α-HSD mRNA is one of the most important reasons for delayed parturition of SCNT calf. (poster)
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