Abstract
Theruntlocus inDrosophilaencodes a nuclear protein involved in embryo segmentation, sex determination/X dosage compensation, and neurogenesis.runthomologues have been identified in higher vertebrates. The encoded proteins share a domain of 128 amino acids called the runt domain. It has been reported that this domain mediates DNA binding and heterodimerization. Here, we analyzeruntBexpression during chondrocyte differentiation “in vitro” and “in vivo.” We have first isolated, from a chondrocyte library, a cDNA clone coding for aruntBchicken homologue and containing a complete open reading frame. The predicted protein product is 84% identical to the mouse PEBP2αB2 isoform. By RT-PCR analysis we have also cloned the chicken cDNA fragment coding for ΔαB2, the exon sequence included in the B1 isoform mRNA. On Northern blot analysis of cultured chondrocytes, runtB mRNA levels increase dramatically with the transition from stage 0 (dedifferentiated) to stages I and II (hypertrophic chondrocytes). Moreover, runt polypeptides were demonstrated in chondrocytes bothin vivoandin vitro.These results suggest thatruntplays a role in chondrogenic differentiation.
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