Expression of Y-box-binding protein-1 and epithelial-mesenchymal transition markers in colorectal cancer

Abstract

Objective To detect the expressions of Y-box-binding protein-1（YB-1）and epithelial-mesenchymal transition（EMT）markers（E-cadherin and N-cadherin）in colorectal cancer（CRC）,to analyze the relationship between the expression of YB-1 and clinicopathological parameters,to evaluate the correlations among YB-1,E-cadherin and N-cadherin. Methods The expressions of YB-1,E-cadherin and N-cadherin in 120 primary CRC tumors and corresponding normal tissues were detected by western blot and immunohistochem-istry and the results were analyzed. Results The expressions of YB-1,E-cadherin and N-cadherin in tumors were significantly different from those in corresponding normal tissues（χ2 = 47. 373,P ﹤ 0. 05;χ2 = 83. 145, P ﹤ 0. 05;χ2 = 41. 832,P ﹤ 0. 05）. The expression of YB-1 in tumors was associated significantly with tumor differentiation,tumor invasion,lymph node metastasis and distance metastasis（χ2 = 8. 077,P = 0. 008;χ2 =8. 178,P = 0. 006;χ2 = 15. 152,P ﹤ 0. 001;χ2 = 7. 368,P = 0. 011）. It was negatively correlated with E-cadherin expression（r = - 0. 238,P = 0. 009）,but positively correlated with N-cadherin expression（r =0. 361,P ﹤ 0. 001）. Conclusion YB-1 may promote the occurrence and development of CRC by participating in EMT program. Key words: Y-box-binding protein 1 ; Colorectal neoplasms ; Epithelial-mesenchymal transition