Abstract
Objective: Increasing evidence indicates that prostate cancer (PCa) cells possess voltage-gated sodium channels (VGSCs) and these channels contribute to the metastatic progression of the disease. In the present study, it was aimed to investigate the heterotopic expression of VGSCs in carcinogenesis and metastasis. It was evaluated whether the expression of VGSC in strongly metastatic rat Mat-LyLu PCa cells observed in vitro is also conserved when these cells form primary tumors and metastasize to the lungs upon their subcutaneous injection in rats. Methods: PCa model was induced in male Copenhagen rats by subcutaneous implantation of strongly metastatic Mat-LyLu cells. The expression of VGSCs was studied in the model utilizing molecular biological and immunochemical techniques. Results: Reverse transcription PCR and immunohistochemistry showed that the expression of VGSC is conserved in the primary prostate tumors of Mat-LyLu and not eliminated in the corresponding lung metastases at both the mRNA and protein level. Conclusion: The results are consistent with the predominant role of VGSC in the metastatic progression of PCa and consistently support the VGSC hypothesis of cancer progression. J Clin Exp Invest 2013; 4 (4): 422-428
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