Expression of VEGFR-3 in the lymphatic vessels of the human gum lamina proper within the age and inflammation

Abstract

Activation of vascular endothelial growth factor VEGFR-3 leads to proliferation, differentiation and migration of lymphatic endothelial cells. The role of this factor in periodontal tissues in normal and inflammatory conditions requires extensive study. The purpose of this study: to study the distribution and expression of VEGFR-3 in the lymphatic vessels of the gum lamina proper during inflammation and the use of a diode laser in young and mature people. Slices of human gum biopsy material were studied by immunohistochemical method. As a result of the research , it was found that in the patients with conditionally healthy periodontal tissues, the number of lymphatic vessels depends on the age and differs in young patients compared to mature patients by 1.2 times. Simultaneouslyбол, it was found that in the control groups, the majority of vessels do not contain VEGFR-3 or have weak receptor expression regardless of age. The tendency to VEGFR-3-mediated increasing of the number of lymphatic vessels during the inflammation is observed only at the age of 41 to 60, however, the intensity of the expression of the VEGF type 3 receptor in chronic gingival inflammation shifts towards the increasing of the vessels with medium and strong VEGFR-3 expression in both groups. As a result of the study, it was found that the tendency to change the number of VEGFR-3-positive vessels is observed only in inflamed gum tissue in patients after 41. Diode laser has a stimulating effect on VEGFR-3-mediated vascular circulation in young patients, while in mature patients, diode laser therapy leads to increase the part of lymphatic vessels with medium and strong expression of the studied receptor, but practically does not affect on the number of detected vessels.