Expression of vascular relevant factors in rabbits with deep II degree burn based on the Notch signaling pathway

Abstract

To investigate the relationship between the Notch signaling pathway and expression of vascular relevant factors in rabbit deep II degree burn model. Methods: A total of 120 New Zealand white rabbits were randomly divided into a block group, a model group, and a control group. The block group was injected 2 mg/kg γ-secretase inhibitor (GSI) once a day at 1 d before the model establishment, and 1-14 d after the deep II degree burns model establishment. The model group were injected physiological saline at the same time. The control group was only injected with the same amount of saline. The expressions of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR-2), matrix metalloprotein 2 (MMP-2) and matrix metalloprotein 9 (MMP-9) were detected by immunohistochemistry. Results: The expressions of VEGF and VEGFR-2 in the model group and the block group were significantly increased within 21 days after modeling, while decreased after 21 days; the expressions of MMP-2 and MMP-9 were decreased within 21 days after modeling, while increased after 21 days, with significant differences compared with the control group (P<0.05). The expressions of VEGF and VEGFR-2 in the model group were higher than that in the block group, and the expressions of MMP-2 and MMP-9 were lower than those in the block group (P<0.05). The expression of VEGFR-2 was positively correlated with VEGF, while MMP-2 and MMP-9 were negatively correlated with VEGF within 21 days after modeling. Conclusion: In the rabbit deep II degree burn model, the Notch signaling pathway was blocked to attenuate the expressions of VEGF and VEGFR-2, and to up-regulate the expressions of MMP-2 and MMP-9.