Expression of Vascular Endothelial Growth Factor (VEGF) and New Blood Vessels Formation on Wound Incision Post Ropivacaine Administration in Animal Model

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Surgical incision wounds causing clinical pain. Inadequate postoperative pain management resulting in impaired wound healing. A critical factor in wound healing is angiogenesis. VEGF is one of the important factors and regulators of angiogenesis. The aim of this study is to analyze the effect of infiltration ropivacaine on VEGF expression, and the formation of new blood vessels, which is very instrumental in the wound healing process. Methods: Twenty-four Wistar rats were divided into two groups, and then superficial-thickness excisional wounds were created. One was treated by ropivacaine infiltration, and the other was not given ropivacaine infiltration as control group. Each of the groups was divided into two subgroups. Each of the subgroups composed of 6 Wistar rats based on the period of termination: 3rd, 7th day after wounding. Histological evaluation was done to determine the presence of new small blood vessels and the expression of VEGF of capillary endothelial cells. The data collected is then analysed by SPSS. Results: The administration of ropivacaine infiltration on superficial-thickness excisional wounds improved wound healing characterized by neovascularization formed and changed of expression of VEGF within cytoplasm or surface of endothelial cells Conclusion: The administration of ropivacaine infiltration has a beneficial effect on the healing process of superficial Wistar rats.