Expression of tumor-infiltrating lymphocyte CD3 and human leukocyte antigen-DR in colorectal carcinoma and their prognostic significance

Abstract

Objective To explore the expression of tumor-infiltrating lymphocyte CD3 and human leukocyte antigen-DR (HLA-DR) in colorectal carcinoma and their prognostic significance.Methods Immunohistochemical technique (IHCA) and flow cytometry (FCM) were used to detect the expression of tumor-infiltrating lymphocyte CD3 and HLA-DR in 60 cases of colorectal carcinoma.The relationship of their expression with clinico-pathological features and prognosis of colorectal carcinoma was analyzed.Results The expression rate of CD3 and HLA-DR was respectively 38.33％ (23/60) and 41.67％ (25/60).The expression rate of CD3 and HLA-DR was correlated with tumor Dukes stage,differentiation degree of tumor and prognosis of patients,but not with patients' gender,age and lymph node metastasis.The expression of CD3 and HLA-DR was significantly increased in well-differentiated tumor group as compared with low-differentiated tumor group,and decreased with increases in Dukes staging.The expression levels of CD3 and HLA-DR were significantly higher in ≥ 5-year survival group than ＜ 3-year denth group ( P ＜0.05).Flow cytometry revealed the expression of CD3 and HLA-DR was significantly higher in ≥ 5-year survival group than in ＜ 3-year denth group ( P ＜ 0.05 ).The expression of CD3 and HLA-DR had no correlation with patients' gender,age,lymph node metastasis and differentiation degree of tumor.Conclusion TIL CD3 and HLA-DR are the important characteristic markers in colorectal carcinoma,and can be used to prodict the prognosis. Key words: Colorectal Carcinoma; CD3; Human leukocyte antigen; Prognosis