Abstract
Objective: To investigate the expression of TRAIL receptors in human hepatocellular carcinoma and neighboring non-tumor liver tissues and to study its roles in apoptosis of SK-Hep1 cell line.Methods: The expression of TRAIL receptors in hepatocellular carcinoma and in SK-Hep1 cell line was detected by immunohistochemical staining and RT-PCR, and the apoptosis of SK-Hep1 cell line induced by recombinant human TRAIL together with or without INF-γ was measured by FCM.Results: The expression of four TRAIL receptors was detectable in human hepatocellular carcinoma and neighboring non-tumor liver tissues. High expression of DR4 and DR5 was detected in tumor and neighboring non-tumor liver tissues whereas low expression of DcR1 and DcR2 was found in tumor, significantly lower than that in normal liver tissues (67.86% vs 100%, 78.57% vs 100%, respectively,P<0.01. TRAIL together with INF-γ strongly increased the apoptosis of SK Hep1 cell line.Conclusion: The four TRAIL receptors can be detected in hepatocellular carcinoma and in SK-Hep1 cell line. Combinations of TRAIL and INF-γ may be a useful alternative to HCC.
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