Expression of TRAF6 mRNA on the Resorbed Surface of Deciduous Teeth Root

Abstract

The replacement of deciduous teeth by permanent teeth was an in vivo controlled physiological course. Odontoclast is the main functional cell involved the physiological root resorption of human primary teeth. TRAF6 is the key molecule in the signals regulation during the procedure of differentiation and maturity of osteolasts/odontoclasts. This experiment was to study the expression of TRAF6 in physiological root resorption of deciduous and permanent teeth. In addition, the effect of TRAF6 during the physiological root resorption of human deciduous teeth was also discussed. Permanent and deciduous teeth in physiological resorption period were decalcified, embedded and sliced. TRAF6 antibody and TRAF6 in situ hybridization agent were used to detect samples’ expression of TRAF6 protein and mRNA. Im-pro-plas 6.0 analytical system was used to measure the photodensity of odontoclasts, odontoblasts and dental pulp fibroblasts respectively between study and control group. It was observed that TRAF6 protein and mRNA of odontoclasts, odontoblasts and dental pulp fibroblasts existed in deciduous teeth with physiological resorption showing positive stained results. However, in permanent tooth, odontoclast was not observed and TRAF6 in odontoblast and dental pulp fibroblasts displayed negative. For the same cells of the two groups, t-test showed that p‹0.01. The optical density of the deciduous teeth odontoclasts was higher than that of the odontoblasts and dental pulp fibroblasts, P‹0.01; the odontoblasts and dental pulp fibroblasts showed no difference. TRAF6 activated the root resorption of deciduous teeth by participating in cellular differentiation and function.