Expression of TNFalpha and TGFbeta1 in Guillain-Barré syndrome: correlation of a low TNFalpha-/TGFbeta1-mRNA ratio with good recovery and signs for immunoregulation within the cerebrospinal fluid compartment.

Abstract

In the pathogenesis of Guillain-Barré syndrome (GBS) a dysregulation of cytokines is supposed. The protein concentration and mRNA expression of TNFalpha and TGFbeta1 were investigated in cerebrospinal fluid (CSF) and blood in 10 patients with GBS. TNFalpha-mRNA was increased at the beginning of the disease and demonstrated a decline during the time course (P = 0.001). The level of TNFalpha protein was elevated in only a few patients. TGFbeta1-mRNA (P = 0.001) and the active TGFbeta1 protein (P < 0.05) increased during the course of GBS, and the level of total TGFbeta1 protein was temporarily elevated (P = 0.005). A low ratio of TNFalpha-/TGFbeta-mRNA expression correlated to a good clinical course (P < 0.05). The results indicate an association of TNFalpha with disease activity. TGFbeta1 seems to terminate and limit the inflammatory reactions and to promote the healing course of GBS. In addition the investigations show that in GBS immunoregulatory mechanisms also take place in the CSF compartment itself and that CSF cells are involved in the production of pro-inflammatory as well as immunosuppressive cytokines.